expression plasmid pmscv puro ires gfp 64 (Addgene inc)
Addgene inc is a verified supplier 
93
Structured Review
Addgene inc
expression plasmid pmscv puro ires gfp 64
Expression Plasmid Pmscv Puro Ires Gfp 64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmscv+pig+vector/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/10__1016_slash_j__isci__2026__115182-641-41-45
Average 93 stars, based on 77 article reviews
Expression Plasmid Pmscv Puro Ires Gfp 64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmscv+pig+vector/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/10__1016_slash_j__isci__2026__115182-641-41-45
Average 93 stars, based on 77 article reviews
expression plasmid pmscv puro ires gfp 64 - by Bioz Stars,
2026-09
93/100 stars
Images
Related Articles
Clone Assay:Article Title: MiR-137 affects melanin synthesis in mouse melanocyte by repressing the expression of c-Kit and Tyrp2 in SCF/c-Kit signaling pathway. Article Snippet: Mouse pre-miR-137 and a partial 3′UTR of mouse c-Kit gene with the miR-137 binding site were, respectively, amplified and cloned into the pUC-T vector (Takara, Biotechnology Co., Ltd.). .. The cloned fragment of pre-miR-137 was subsequently subcloned into Article Title: Ocular Albinism Type 1 Regulates Melanogenesis in Mouse Melanocytes Article Snippet: Mouse melanocytes were cultured in melanocyte medium (MelM) (ScienCell Research Laboratories, Carlsbad, CA, USA) supplemented with 1% melanocyte growth supplement (MelGS) and 1% penicillin-streptomycin and 0.5% fetal bovine serum at 37 °C in a humidified 5% CO 2 atmosphere. .. The mice OA1 gene was PCR amplified and then was cloned into the Article Title: The long noncoding RNA CHROME regulates cholesterol homeostasis in primate Article Snippet: For transient knockdown of CHROME , primary human hepatocytes, HepG2 cells or PMA-differentiated THP-1 cells were transfected with 62.5 nM locked nucleic acid (LNA) GapmeRs targeting a common regions of all CHROME variants or Negative Control A (Exiqon) using Lipofectamine RNAiMax (Life Technologies). .. To create CHROME -overexpressing cell lines, CHROME cDNAs were obtained from Genscript and cloned into Article Title: The Regulation of COX-2, ABCA1 and ABCG1 by the lncRNA PACERR links the inflammatory response and cholesterol homeostasis Article Snippet: Cells stably expressing the shRNAs were selected by using 5 μg ml −1 puromycin (Thermo Fisher Scientific). .. To create PACERR-overexpressing cell lines, PACERR gene block from IDT was cloned into the Article Title: A long non-coding RNA targets microRNA miR-34a to regulate colon cancer stem cell asymmetric division Article Snippet: The full length of Lnc34a was then amplified using the primers: 5`-TTAACCAGTCGGCCTTCCTCGCC-3` and 5`-TGAGATTAACCGACTTTCCCAAG-3`, then cloned into pGEM-T (Promega, Durham, NC) for sequencing. .. The full length of Lnc34a was cloned into Article Title: The long non-coding RNA LUCAT1 is a negative feedback regulator of interferon responses in humans Article Snippet: LUCAT1 was cloned from 5′ RACE cDNA using Q5 Polymerase (NEB, Cat#M0491) with a 5′-specifc primer with a XhoI recognition site (5′-ataccgctcgagAATCAACACTCCACTCAGACAATGCC-3′) and two different 3′-specifc primer with EcoRI recognition sites (Primer for the short isoform: 5′aggaattcTGAGACAGAGTCTCACTCTGTTGCC-3′; and primer for the long isoform: 5′aggaattcGTATCTGCCTTTTCAGGCAGTGAAATC-3′). .. The amplicons were cloned into Polymerase Chain Reaction:Article Title: Ocular Albinism Type 1 Regulates Melanogenesis in Mouse Melanocytes Article Snippet: Mouse melanocytes were cultured in melanocyte medium (MelM) (ScienCell Research Laboratories, Carlsbad, CA, USA) supplemented with 1% melanocyte growth supplement (MelGS) and 1% penicillin-streptomycin and 0.5% fetal bovine serum at 37 °C in a humidified 5% CO 2 atmosphere. .. The mice OA1 gene was PCR amplified and then was cloned into the Amplification:Article Title: Ocular Albinism Type 1 Regulates Melanogenesis in Mouse Melanocytes Article Snippet: Mouse melanocytes were cultured in melanocyte medium (MelM) (ScienCell Research Laboratories, Carlsbad, CA, USA) supplemented with 1% melanocyte growth supplement (MelGS) and 1% penicillin-streptomycin and 0.5% fetal bovine serum at 37 °C in a humidified 5% CO 2 atmosphere. .. The mice OA1 gene was PCR amplified and then was cloned into the Transfection:Article Title: The long noncoding RNA CHROME regulates cholesterol homeostasis in primate Article Snippet: For transient knockdown of CHROME , primary human hepatocytes, HepG2 cells or PMA-differentiated THP-1 cells were transfected with 62.5 nM locked nucleic acid (LNA) GapmeRs targeting a common regions of all CHROME variants or Negative Control A (Exiqon) using Lipofectamine RNAiMax (Life Technologies). .. To create CHROME -overexpressing cell lines, CHROME cDNAs were obtained from Genscript and cloned into Article Title: The Regulation of COX-2, ABCA1 and ABCG1 by the lncRNA PACERR links the inflammatory response and cholesterol homeostasis Article Snippet: Cells stably expressing the shRNAs were selected by using 5 μg ml −1 puromycin (Thermo Fisher Scientific). .. To create PACERR-overexpressing cell lines, PACERR gene block from IDT was cloned into the Virus:Article Title: Effective inhibition of miR-330/SHIP1/NF-κB signaling pathway via miR-330 sponge repolarizes microglia differentiation. Article Snippet: Neuroinflammation mediated by microglia has been identified as a vital pathogenesis in Parkinson’s disease (PD).. This study aimed to investigate the role and potential regulatory mechanism of microRNA-330 in the LPS-induced chronic neuroinflammatory model. Primary microglia chronic inflammation model and PD animal model were established by LPS treatment.. Bulged microRNA-330 sponges containing 6 microRNA binding sites were constructed and delivered by plasmid or rAAV2/5-GFP vector. Cloning:Article Title: Effective inhibition of miR-330/SHIP1/NF-κB signaling pathway via miR-330 sponge repolarizes microglia differentiation. Article Snippet: Neuroinflammation mediated by microglia has been identified as a vital pathogenesis in Parkinson’s disease (PD).. This study aimed to investigate the role and potential regulatory mechanism of microRNA-330 in the LPS-induced chronic neuroinflammatory model. Primary microglia chronic inflammation model and PD animal model were established by LPS treatment.. Bulged microRNA-330 sponges containing 6 microRNA binding sites were constructed and delivered by plasmid or rAAV2/5-GFP vector. Blocking Assay:Article Title: The Regulation of COX-2, ABCA1 and ABCG1 by the lncRNA PACERR links the inflammatory response and cholesterol homeostasis Article Snippet: Cells stably expressing the shRNAs were selected by using 5 μg ml −1 puromycin (Thermo Fisher Scientific). .. To create PACERR-overexpressing cell lines, PACERR gene block from IDT was cloned into the Plasmid Preparation:Article Title: The Regulation of COX-2, ABCA1 and ABCG1 by the lncRNA PACERR links the inflammatory response and cholesterol homeostasis Article Snippet: Cells stably expressing the shRNAs were selected by using 5 μg ml −1 puromycin (Thermo Fisher Scientific). .. To create PACERR-overexpressing cell lines, PACERR gene block from IDT was cloned into the Expressing:Article Title: A long non-coding RNA targets microRNA miR-34a to regulate colon cancer stem cell asymmetric division Article Snippet: The full length of Lnc34a was then amplified using the primers: 5`-TTAACCAGTCGGCCTTCCTCGCC-3` and 5`-TGAGATTAACCGACTTTCCCAAG-3`, then cloned into pGEM-T (Promega, Durham, NC) for sequencing. .. The full length of Lnc34a was cloned into Transformation Assay:Article Title: The long non-coding RNA LUCAT1 is a negative feedback regulator of interferon responses in humans Article Snippet: LUCAT1 was cloned from 5′ RACE cDNA using Q5 Polymerase (NEB, Cat#M0491) with a 5′-specifc primer with a XhoI recognition site (5′-ataccgctcgagAATCAACACTCCACTCAGACAATGCC-3′) and two different 3′-specifc primer with EcoRI recognition sites (Primer for the short isoform: 5′aggaattcTGAGACAGAGTCTCACTCTGTTGCC-3′; and primer for the long isoform: 5′aggaattcGTATCTGCCTTTTCAGGCAGTGAAATC-3′). .. The amplicons were cloned into |